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KMID : 0376419780020000195
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Chonbuk University Medical Journal
1978 Volume.2 No. 0 p.195 ~ p.201
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Studies on Rat Stomach Alcohol Dehydrogenase
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Abstract
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The enzymatic properties of rat stomach alcohol dehydrogenase was characterized in comparison to the liver alcohol dehydrogenase and the physiological role of this enzyme in the metabolsim of ethanol was also studied.
Stomach alcohol dehydrogenase was different from that of liver in Km for ethanol, substrate specificity, and temperature optimum. The optimal temperature of stomach enzyme was 55C, much higher than that of liver enzyme, while both of them attained the maximal activity at pH 11. The stomach enzyme had a stronger of `inity to aromatic alcohols such as benzyl and furfuryl alcohol than ethanol. The Micha ~lis constant of stomach alcohol dehydrogenase for ethanol was 2120 mM, abput 700 times higher than that of liver alcohol dehydrogenase, whereas they had´ comparable affinity to NAD.
NAD concentrations in the stomach and liver were much less than the Km values for NAD of alcohol dehydrogenases from those organs, suggesting that NAD is an important regulatory factor in the metabolism of ethanol. The constant rate of ethanol clearance regardless of its concentration in b´-ood together with the limited content of NAD and the low total activity of ADH in the stomach indicates that alcohol dehydrogenase of rat stomach cannot be expected to play a significant role in the metabolism of ethanol
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